The EMBO Journal (2018)
Zaffagnini, G., Savova, A., Danieli, A., Romanov, J., Tremel, S., Ebner, M., Peterbauer, T., Sztacho, M., Trapannone, R., Tarafder, A.K., Sachse, C., and Martens, S.
In this paper, we show that in vitro the autophagy receptor p62 and ubiquitinated substrates spontaneously phase separate into clusters. Mechanistically, this is based on the crosslinking of p62 filaments by the substrates. The cargo receptor NBR1 directly stimulates this process. We further uncover multiple modes of regulation of the clustering reaction that suggest how this process can be integrated into general proteostasis and coordinated with autophagosome formation.